Journal: PLOS Pathogens

Article Title: Listeria monocytogenes requires phosphotransferase systems to facilitate intracellular growth and virulence

doi: 10.1371/journal.ppat.1012492

Figure Lengend Snippet: (A) Intracellular growth of WT, Δ glpD /Δ golD , Δ uhpT, Δ glpD /Δ golD /Δ uhpT was determined in BMDMs following infection at an MOI of 0.2. Growth curves are representative of at least three independent experiments. Error bars represent the standard deviation of the means of technical triplicates within the representative experiment. (B) L2 fibroblasts were infected with indicated L. monocytogenes strains at an MOI of 0.5 and were examined for plaque formation 4 days post infection. Assays were performed in biological triplicate and data displayed is the median and SEM of a strain’s plaque size relative to WT in one of three representative biological replicates. (C) Bacterial burdens from the spleen and liver were enumerated at 48 hours post-intravenous infection with 1x10 5 bacteria. Data are representative of results from two separate experiments. Horizontal dashed lines represent the limits of detection, and the bars associated with the individual strains represents the mean and SEM of the group.

Article Snippet: Plaque assays were conducted using a L2 fibroblast cell line grown in Dulbecco’s Minimal Essential Media (DMEM) based media (Thermo Fischer: 11965092) as previously described with minor modifications for visualization and quantification of plaques [ , ].

Techniques: Infection, Standard Deviation, Bacteria

Journal: PLOS Pathogens

Article Title: Listeria monocytogenes requires phosphotransferase systems to facilitate intracellular growth and virulence

doi: 10.1371/journal.ppat.1012492

Figure Lengend Snippet: (A) PTS mediated free sugar import and phosphorylation by phosphocarrier protein phospho-cycling from the terminal conversion of phosphoenol-pyruvate (PEP) to pyruvate. (B) Intracellular growth of WT, Δ glpD /Δ golD /Δ uhpT , Δ ptsI , and Δ glpD /Δ golD /Δ uhpT /Δ ptsI was determined in BMDMs following infection at an MOI of 0.2. Growth curves are representative of at least three independent experiments. Error bars represent the standard deviation of the means of technical triplicates within the representative experiment. (C) L2 fibroblasts were infected with indicated L. monocytogenes strains at an MOI of 0.5 and were examined for plaque formation 4 days post infection. Assays were performed in biological triplicate and data displayed is the median and SEM of a strain’s plaque size relative to WT in one of three representative biological replicates. (D) Bacterial burdens from the spleen and liver were enumerated at 48 hours post-intravenous infection with 1x10 5 bacteria. Data are representative of results from two separate experiments. Horizontal dashed lines represent the limits of detection, and the bars associated with the individual strains represents the mean and SEM of the group.

Article Snippet: Plaque assays were conducted using a L2 fibroblast cell line grown in Dulbecco’s Minimal Essential Media (DMEM) based media (Thermo Fischer: 11965092) as previously described with minor modifications for visualization and quantification of plaques [ , ].

Techniques: Phospho-proteomics, Infection, Standard Deviation, Bacteria